FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHmock24_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHmock24_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20288534
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT49550.011.0262261
ACGGTTA23950.09.5141523
GCGGTAT23900.09.1439251
CCGGGAT63600.09.0385961
CGGGATT38950.09.0260341
CGCCAAT37300.08.91583251
CGGTTAG29350.08.7345254
CGGGGAT73450.08.7320191
CGGGAAT47550.08.6924341
AATCGAA39200.08.4778335
GCGGGAT49700.08.4119951
CGCGGAT33300.08.1321121
CGGGTAT25750.08.1179841
CGGATCG15303.6379788E-128.0670783
GCGGGTT38900.08.0605951
CGGGGTT67500.08.02368451
CGCGGTT26550.07.87337351
CGCGAAT12803.2869139E-97.79438831
GGGAAAT140300.07.7883041
GCCGAAT40350.07.77093171
Produced by FastQC (version 0.11.5)