FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHmock12_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHmock12_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36841720
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG568020.15417846940913726No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC368760.1000930466872882Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA78650.026.54436562-63
GCCGTAT87750.024.30565560-61
CGCCGTA92250.023.14509858-59
CCGTATC98850.021.36009460-61
TCGCCGT114850.018.50792358-59
TGGTCGC120300.018.04323654-55
AAAAGGG758300.016.9884480-81
AAGGGGG793950.016.11751682-83
TAGGGGG292650.015.07200250-51
TAAAAAA1812450.014.50026270-71
AAAGGGG730100.014.306602582-83
GTATCAT149050.014.18208962-63
TTTAGGG223250.013.59916248-49
AAAAAAG1591350.013.51017778-79
GTCGCCG167400.013.23654956-57
GGTCGCC168650.013.18068856-57
GGGTTAA154150.012.83463166-67
GTGGTCG178450.012.56288254-55
TCTCGGT162800.012.38870948-49
AAAAAGG1003500.012.18180480-81
Produced by FastQC (version 0.11.5)