FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHmock12_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHmock12_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20390803
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTT38650.011.0628711
CGCGGGT43100.011.0229431
CCGGGAT62400.010.8874521
CGGGGAT66550.010.7082381
GCGGGAT48900.010.0069831
GCGGAAT33750.09.9944611
GCCGATT24100.09.4623511
GCGGTAT21100.09.2315841
CGCGTAT5254.4889934E-49.0493111
CGGGAAT49550.09.0127851
CGGGCAT86250.08.9234081
CGCGAAT12653.274181E-118.6379791
CGGCAAT42100.08.4635771
GGCAAAT145750.08.377211
GCCGGAT52150.08.1079391
CGATAAC23750.08.0014961
CGGGTAT22350.07.86500551
CGGGATT35800.07.82456732
GTCCGAT18950.07.7719021
ATCGAAC21400.07.7650756
Produced by FastQC (version 0.11.5)