FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHmock06_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHmock06_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27065928
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA35250.013.61081862-63
GCCGTAT42850.012.13894260-61
AAGGGGG366850.011.26505882-83
AAAAGGG367850.011.13139680-81
TAGGGGG141250.011.03153250-51
CGCCGTA49500.010.93971458-59
GCGGGAT66400.010.3749631
GCGGTAT28200.010.1085481
CGGGGAT91900.09.9259551
GCGGGTT50750.09.9233071
CGCGGGT58250.09.7875031
GCGGAAT50050.09.6823921
CCGTATC56300.09.61864760-61
CCGGGAT84000.09.3323551
CGCGATT10254.2382453E-109.2702781
TAAAAAA873600.09.10546570-71
CGGGGTT91000.09.0321531
GTCGGAT32850.08.9669121
CGGGTAT28150.08.7763011
AAAGGGG343000.08.72467282-83
Produced by FastQC (version 0.11.5)