FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHmock06_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHmock06_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13098138
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT14050.011.8357831
GCGGGTT24400.011.6833311
GCGGAAT24400.011.0991641
CGGGGTT43500.010.15778351
CTCGGTT23200.09.4205251
CGCGGGT26700.09.2533151
GCGGCAT34250.09.1556381
CCGCAAT19250.09.1322171
CGGGGAT42850.09.0921851
GTAATCG8702.4324981E-78.7304063
GCGGTTT25450.08.4009811
CGGGTCT50000.08.3549993
CCGGAAT30750.08.3436081
GCGGGAT29050.08.3412151
GTCGATT10304.1854946E-88.3031051
GTCCGAT12101.0950316E-98.2459211
CCGGGAT39950.08.2061151
CGGGTGT42750.08.1131961
CGCCAAT24050.08.0997951
ATTAGCG6504.2093638E-48.0350336
Produced by FastQC (version 0.11.5)