FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23332343
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15161886.4982243746373864No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG367710.1575966888537512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA140250.034.32801442-43
AGAGCAC659350.033.579578
CTCGTAT139150.033.40537344-45
ATGCCGT162000.032.47447248-49
ATCTCGT147950.030.64799142-43
GAGCACA739200.030.1523259
TATCTCG54750.029.74559840-41
GTCACGA68750.029.6993928-29
CGGAAGA779250.028.9640054
TCGGAAG792000.028.3524533
AATCGGG63750.028.00401740-41
ATCGGAA821250.027.5354962
CAGTCAC321650.027.11083426-27
CCAGTCA346400.026.98317326-27
AAGAGCA841750.026.9629697
GATCGGA833850.026.472121
CTCCAGT393650.026.47019224-25
CGTATCT29850.026.24531738-39
GCTTGAA196200.026.22366360-61
ACTCGAT49650.025.91579636-37
Produced by FastQC (version 0.11.5)