FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHy24_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHy24_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24469267
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2087580.8531436597589948TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC271600.042.0079246-47
TATGCCG273550.041.8820248-49
CTCGTAT268000.041.54431544-45
TCGTATG268800.040.85520644-45
TCGTATC298800.040.41731338-39
CGTATCT285300.039.58273738-39
TATCTCG285250.039.0902840-41
TCTCGTA278400.038.8833742-43
ATCTCGT298450.037.56827542-43
GTATGCC314450.036.14756446-47
ATTCGTA351400.036.11126336-37
AATTCGT349700.035.81852734-35
TTCGTAT341750.035.72714636-37
CACGAAT361200.035.09916330-31
ATGCCGT317850.034.9762748-49
ACGAATT371700.034.7466232-33
GCCGTCT299800.034.6263150-51
TGCCGTC321100.034.54829450-51
CCGTCTT314950.034.03147552-53
GAATTCG377650.033.86565434-35
Produced by FastQC (version 0.11.5)