FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHy24_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHy24_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16769275
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1463950.8729954037965266TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG208750.039.27361348-49
CGTATGC213550.038.60215446-47
CTCGTAT216550.037.49707844-45
TCGTATG214200.037.0436344-45
TATCTCG227750.034.4643140-41
TCTCGTA227200.034.29686742-43
GTATGCC238500.033.3988346-47
ATCTCGT246800.032.90110442-43
GCCGTCT236700.032.90028850-51
TGCCGTC248900.032.73801450-51
CCGTCTT250050.032.3310152-53
ATGCCGT252450.031.33688248-49
AGGGGGG203550.028.90090674-75
CTTGAAA285550.028.40305762-63
CGCTCAT294650.028.35682534-35
TGCTTGA290350.027.49180260-61
ACCGCTC305300.027.38343632-33
GCTTGAA287600.027.19314260-61
CATTATC301450.027.10993838-39
CTCATTA313350.026.853636-37
Produced by FastQC (version 0.11.5)