FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHy12_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHy12_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25825518
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4347991.6836022417827206TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG567800.042.5846748-49
CGTATGC569900.042.5278946-47
CTCGTAT570700.041.6610644-45
TCGTATG569350.040.45419744-45
ATCTCGT603700.039.3562142-43
CCGTCTT605050.039.08760552-53
TCTCGTA586000.038.5144642-43
TATCTCG587450.038.44365340-41
TGCCGTC630550.038.11327750-51
ATGCCGT609450.037.70648248-49
GCCGTCT599900.037.57829750-51
GTATGCC618200.037.39571446-47
CGCTCAT693900.035.1850934-35
CATTATC684250.034.95213338-39
ACCGCTC699750.034.86401732-33
CTTGAAA690500.034.3811862-63
TTATCTC695000.034.23394840-41
CGTCTTC661300.034.04615452-53
CTCATTA722400.033.9479436-37
TGCTTGA706300.033.13794760-61
Produced by FastQC (version 0.11.5)