FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHy12_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHy12_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23036229
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2038910.885088440473482TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC263850.041.81068446-47
TATGCCG266350.041.66777848-49
TCGTATG260200.040.65397344-45
CTCGTAT266500.040.56627744-45
TCGTATC299950.039.47075738-39
TATCTCG280600.038.43465440-41
CGTATCT286700.038.22161538-39
TCTCGTA280400.037.43724442-43
ATCTCGT294200.037.1019742-43
ATTCGTA334100.036.96463836-37
TTCGTAT327500.036.17943636-37
ATGCCGT306000.035.1665648-49
AATTCGT345900.035.0034834-35
GTATGCC312000.034.92428246-47
GCCGTCT289950.034.61487250-51
ACGAATT364600.034.4264832-33
CCGTCTT303350.034.3149852-53
GAATTCG363500.034.15152434-35
CACGAAT359700.034.05048830-31
TGCCGTC322900.033.56865350-51
Produced by FastQC (version 0.11.5)