FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHy06_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHy06_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24200950
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3197991.3214315966935182TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG394950.043.62695748-49
CGTATGC392200.043.5998946-47
CTCGTAT389100.042.78147544-45
TCGTATG388700.042.23893444-45
TCGTATC429400.042.20093538-39
TATCTCG403500.041.20765740-41
CGTATCT411700.041.17705538-39
ATTCGTA464900.040.89420736-37
TCTCGTA397950.040.5469742-43
TTCGTAT455600.040.0921336-37
ATCTCGT423350.039.47186742-43
AATTCGT483800.038.73227334-35
ACGAATT500500.038.46029332-33
ATGCCGT435150.038.40680348-49
CACGAAT495900.038.1418430-31
GTATGCC446900.038.0932646-47
GAATTCG503350.038.0300434-35
CCGTCTT426100.037.5280352-53
CGAATTC507900.037.16110632-33
GTCACGA510000.037.11546728-29
Produced by FastQC (version 0.11.5)