FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHy06_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHy06_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20192050
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT882490.4370482442347359TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC133850.035.80653446-47
TATGCCG135800.035.36224448-49
TCGTATG133400.034.96602244-45
CTCGTAT137800.034.0046644-45
TATCTCG152100.029.9019940-41
TCTCGTA153850.029.57730342-43
ATCTCGT164550.028.59217342-43
ATGCCGT170350.027.49306748-49
GTATGCC172900.027.19754446-47
CCGTCTT174450.026.98312252-53
GCCGTCT177250.025.91371750-51
TGCCGTC198250.024.21099550-51
ACCGCTC214700.022.45587332-33
CGTCTTC205700.022.38736752-53
CGCTCAT222000.021.76011734-35
TTATCTC221250.021.43657140-41
CATTATC226450.020.89177938-39
ATTATCT221400.020.7783238-39
CTCATTA235050.020.75389936-37
CTTGAAA241000.019.80785462-63
Produced by FastQC (version 0.11.5)