FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHv24_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHv24_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28770932
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4271571.4846825260996064TruSeq Adapter, Index 13 (97% over 38bp)
CGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTT1048630.364475506042001No Hit
ATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATT1019550.3543680823408849No Hit
GAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTAC968940.33677741131222305No Hit
TTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTCA949180.3299093682470905No Hit
CTGACATTGATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAA948730.3297529603837651No Hit
CTCAATTATTATGTGGTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCC940800.32699670625894217No Hit
GCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTT937970.32601307458514034No Hit
CACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGC850640.29565952190912687No Hit
CTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCAT806830.28043234748182644No Hit
GCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCC798920.27768304481759576No Hit
TTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTTAA752670.2616077921980421No Hit
CAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCT700510.2434783829734817No Hit
CAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAA636090.22108772840587856No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT593510.2062880688050008No Hit
TTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTC570870.19841901541458581No Hit
CCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTC552140.1919089725699536No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA521230.18116549022464756No Hit
TTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTGACA359950.1251089120088289No Hit
CACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTC353820.12297828933730753No Hit
GTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCCATCTTCTGTCAATGA349250.12138988059198083No Hit
ATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTTAATGCAA338920.11779945119608916No Hit
GGAGAAGGCAAAGCAGAACTGAGGTATTGAATGACCACATAATAATTGAG317360.11030577667765507No Hit
CCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCA316980.11017369892640251No Hit
CTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTGAC301600.10482802573097041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG557500.042.74950848-49
CGTATGC557200.042.45292346-47
CTCGTAT534850.041.78030444-45
TCGTATG548450.040.7226244-45
ATCTCGT569400.039.62055242-43
TGCCGTC588300.039.21954750-51
TCTCGTA548400.039.17159342-43
CCGTCTT573800.038.8447552-53
GTATGCC599000.038.49920746-47
GCCGTCT555350.038.32201450-51
ATGCCGT591300.038.27750448-49
AATCTCG581200.037.7169240-41
AGGGGGG544700.034.4887774-75
CGTCTTC611750.034.4783552-53
CGCGTAT22600.034.4687544-45
CTTGAAA680150.032.75342662-63
TGCTTGA655300.032.61464760-61
GCTTGAA699500.030.49946860-61
AATCGCG29100.030.3605840-41
ATTCAGA904650.029.41418334-35
Produced by FastQC (version 0.11.5)