FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHv24_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHv24_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27447341
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT3090681.1260398593801855TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT2130300.7761407562211582TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG1004890.36611561025164513TruSeq Adapter, Index 6 (97% over 36bp)
CTCAATTATTATGTGGTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCC720850.26263017608882405No Hit
CGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTT662260.24128384603812805No Hit
CTGACATTGATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAA629780.22945027716892502No Hit
ATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATT580960.21166349046342958No Hit
GCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTT554100.20187747876925494No Hit
CACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGC540460.19690796277861672No Hit
GAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTAC522340.19030623039222633No Hit
CTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCAT508910.185413224545139No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT500360.1822981687005674No Hit
TTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTCA474420.17284734430194895No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT467530.17033708292544622TruSeq Adapter, Index 6 (97% over 36bp)
GCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCC452200.16475184244623187No Hit
TTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTTAA447420.16301032584540703No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA444930.1621031341433037No Hit
CAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCT425790.1551297810596662No Hit
CAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAA414410.15098365994724225No Hit
CCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTC355110.1293786527445409No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT354040.12898881534644832TruSeq Adapter, Index 6 (97% over 36bp)
GTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCCATCTTCTGTCAATGA334060.12170942168860728No Hit
TTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTC301090.10969732915111886No Hit
GGAGAAGGCAAAGCAGAACTGAGGTATTGAATGACCACATAATAATTGAG282790.10303001664168489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG1120400.038.13667340-41
AATCGGG1082650.037.49431640-41
ATCGGGG1014650.037.0906142-43
AGAATCG1224550.035.78130338-39
GAGAATC1391150.034.82699638-39
ATCGGGT133550.034.7844842-43
AATCGCG66250.034.70164540-41
CGGAGAA1544450.033.24155836-37
TCCGGAG1592800.032.53843734-35
CGGGGTT347450.032.41367344-45
CGGGGGT509250.032.11870644-45
AAGGGGG668700.031.84385774-75
CCGGAGA1572250.031.47279234-35
ACTCCGG1673000.030.9618332-33
TCGGGGG694950.030.77448342-43
CTCCGGA1633500.030.49648332-33
GGAGAAT1605450.030.2639936-37
ATTGATT263650.029.0981526
TTGATTA255650.029.0220057
GTTTGCG98400.028.93892148-49
Produced by FastQC (version 0.11.5)