FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHv12_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHv12_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25767371
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT1425260.5531258893272426TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT973050.3776287460602791TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG458960.17811673530838673TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG523300.036.96578240-41
AATCGGG503900.036.1077740-41
ATCGGGG504650.034.13398442-43
AGAATCG582000.033.75576438-39
AATCGCG33550.032.0675140-41
GAGAATC708900.030.93607738-39
CGGAGAA747900.030.8153936-37
TCCGGAG764600.030.51217734-35
ACTCCGG786200.029.7133932-33
CCGGAGA749200.029.4909434-35
CTCCGGA786800.028.34444632-33
CACTCCG803650.027.70291930-31
ATCGGGT75900.026.19046842-43
CGGGGTT202900.025.7045444-45
GTCACTC874000.025.5764728-29
GGAGAAT852050.025.55743836-37
TCGGGGG378800.025.3799142-43
TCACTCC925850.025.3445130-31
CGGGGGT294500.025.32234444-45
AAGGGGG384400.025.14604874-75
Produced by FastQC (version 0.11.5)