FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHv12_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHv12_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21799139
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1314430.6029733559660315TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC188050.039.44180746-47
TATGCCG189850.039.4180848-49
TCGTATG184900.038.00727544-45
CTCGTAT184450.037.95836644-45
TCTCGTA194150.034.7774842-43
ATCTCGT206850.033.97411342-43
AATCTCG207550.033.1500740-41
GTATGCC222400.032.6558446-47
GCCGTCT211700.031.54664250-51
TGCCGTC235450.030.72485750-51
ATGCCGT240850.029.72037548-49
CCGTCTT236350.029.66329852-53
AGGGGGG223300.026.04774-75
CGTCTTC285000.023.466452-53
CGCGTAT9700.023.01520244-45
GTCACAT363800.022.32063528-29
AATCGCG12450.022.12826240-41
CTTGAAA319350.021.81236862-63
AAGGGGG239000.021.42439374-75
CATTCAG381700.021.32977732-33
Produced by FastQC (version 0.11.5)