FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHv06_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHv06_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17729694
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1079390.6088035134729342TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG155550.040.0486348-49
CGTATGC154800.040.0126446-47
CTCGTAT150550.038.99685344-45
TCGTATG151200.038.813544-45
TCTCGTA161700.034.95656642-43
ATCTCGT170800.034.63759242-43
AATCTCG168800.034.0912440-41
GTATGCC187200.032.2246746-47
GCCGTCT180650.031.09228150-51
CCGTCTT190450.030.93892152-53
TGCCGTC200750.030.38077550-51
ATGCCGT196750.030.2017448-49
AGGGGGG193850.025.94893574-75
CGTCTTC224450.024.82377452-53
GTCACAT295600.023.07513628-29
CTTGAAA261200.022.51318262-63
CGCGTAT9800.022.29582644-45
TGCTTGA255150.022.11618860-61
GCTTGAA264850.021.20755460-61
ATTCAGA339750.021.17402834-35
Produced by FastQC (version 0.11.5)