FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHv06_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHv06_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23615683
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT2063250.8736778860048213TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT1411620.5977468447556651TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG672100.2845990099037153TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT304470.12892703547892304TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG702200.039.65296640-41
AATCGGG684550.038.6353440-41
AGAATCG764000.037.58015438-39
ATCGGGG664600.037.0862642-43
GAGCACA1779250.036.6399
AATCGCG43900.036.19280640-41
CGGAGAA929750.035.8030436-37
GAGAATC886500.035.51097538-39
TCCGGAG947000.035.49970234-35
AGAGCAC1880550.034.6319358
ACTCCGG971400.034.61789332-33
CCGGAGA933400.034.50807634-35
CTCCGGA965700.033.57286532-33
CACTCCG973950.033.2567130-31
AAGAGCA2059400.031.6842677
GTCACTC1034100.031.48311828-29
TCACTCC1076450.031.36084230-31
ATCGGGT94400.031.27223242-43
GGAGAAT1021800.031.01574336-37
CGGGGTT235900.030.9482244-45
Produced by FastQC (version 0.11.5)