FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHmock24_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHmock24_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30611819
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT7221972.3592096895646746TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT331980.10844830880517096TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG951750.043.7862948-49
CGTATGC955800.043.7647946-47
CTCGTAT936400.043.0153444-45
TCGTATG948950.041.7231644-45
CCGTCTT984700.041.44319552-53
ATCTCGT936100.040.85990542-43
TCTCGTA915800.040.33151242-43
TGCCGTC1034250.040.0248850-51
GTATGCC1001400.039.86272446-47
ATGCCGT996300.039.79499448-49
GCCGTCT991600.039.30101450-51
CTTGAAA1077950.037.88451862-63
CGTCTTC1027600.037.71848352-53
CATCTCG981250.037.23717540-41
TGCTTGA1098200.036.76424860-61
GCTTGAA1090900.035.66700760-61
ACGAGAT1204550.035.33677732-33
AGGGGGG849450.035.2114674-75
GATTCCA1186800.035.1203536-37
CGAGATT1159750.034.96316532-33
Produced by FastQC (version 0.11.5)