FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHmock24_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHmock24_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20288534
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT906030.44657243347400066TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC143750.036.9753146-47
TATGCCG146800.036.06131448-49
TCGTATG143250.036.0103244-45
CTCGTAT145000.034.87140344-45
TCTCGTA154300.030.83031342-43
TACTCGA171700.030.45848536-37
ACTCGAT167300.029.27210636-37
GTATGCC178750.029.03120246-47
TTACTCG177700.028.94923434-35
ATGCCGT185700.027.91896648-49
CCGTCTT189700.027.605752-53
ATCTCGT175800.027.55967942-43
GCCGTCT197150.026.04452550-51
TGCCGTC210500.025.2953750-51
CTCGATC197550.024.42906638-39
ACATTAC227450.023.77644532-33
ATTACTC226850.023.60879534-35
CGTCTTC224550.022.92998352-53
TCGATCT207050.022.7805538-39
GATCTCG210400.022.55343240-41
Produced by FastQC (version 0.11.5)