FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHmock12_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHmock12_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36841720
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT11983523.2527037282732727TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT479260.13008621747301702TruSeq Adapter, Index 27 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA398130.10806498719386608TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2725500.050.2107939
AGAGCAC2808300.048.7582748
AAGAGCA3010850.045.561517
CGTATGC1557400.045.35389346-47
TATGCCG1560300.045.14316648-49
CTCGTAT1491100.045.06107344-45
CGGAAGA3115000.043.976744
TCGTATG1545900.043.6480844-45
ATCTCGT1463450.043.45389642-43
TCGGAAG3156550.043.364763
TACTCGA1607200.043.32657636-37
CCGTCTT1609800.043.21650352-53
GAAGAGC3176600.043.2076956
TCTCGTA1457350.042.65635342-43
ATCGGAA3223650.042.4074942
TGCCGTC1656100.042.35246350-51
ATGCCGT1610600.041.97118848-49
CTCGATC1525500.041.78906638-39
ACTCGAT1534450.041.7341836-37
GTATGCC1625550.041.72406446-47
Produced by FastQC (version 0.11.5)