FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHmock12_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHmock12_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20390803
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT609330.29882589714588487TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC102850.034.36036746-47
TATGCCG103150.034.05313548-49
TCGTATG101600.033.82478744-45
CTCGTAT103700.033.002444-45
TCTCGTA114050.028.19567942-43
ATCTCGT125450.026.06888642-43
GTATGCC140900.024.6599746-47
ATGCCGT140200.024.59669548-49
CCGTCTT144550.024.15217852-53
GCCGTCT146350.023.40076850-51
CGAGATT167650.021.29248432-33
TGCCGTC167750.021.1517250-51
GTCACGA176700.020.37678728-29
ACGAGAT179850.020.24429332-33
CATCTCG158650.020.20937540-41
TCACGAG182950.020.1348930-31
AGTCACG188100.019.55850628-29
CACGAGA187800.019.09638830-31
CGTCTTC180750.019.03913352-53
GAGATTC225750.016.28586834-35
Produced by FastQC (version 0.11.5)