FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHmock06_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHmock06_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27065928
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3620651.3377150785297294TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC487850.043.30795346-47
TATGCCG490400.042.9857448-49
CTCGTAT481500.042.27620744-45
TCGTATG484250.041.73695444-45
TCTCGTA479350.039.08679242-43
ATCTCGT494900.038.89043442-43
CCGTCTT541150.038.2434952-53
GTATGCC537800.037.94750246-47
ATGCCGT541450.037.53803348-49
GCCGTCT545400.036.6826650-51
TGCCGTC577750.036.3798550-51
CATCTCG535600.034.69357740-41
CGAGATT606150.034.2687132-33
CGTCTTC583200.034.2480752-53
ACGAGAT630250.034.1943532-33
TCACGAG642350.033.73506530-31
GTCACGA628700.033.21331828-29
AGTCACG652200.033.18187328-29
CTTGAAA634150.032.7548162-63
CACGAGA645500.032.40408730-31
Produced by FastQC (version 0.11.5)