FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n01_AHmock06_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n01_AHmock06_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13098138
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT631070.48180130641469804TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC97600.037.6199646-47
TCGTATG95800.037.08725744-45
TATGCCG98250.037.00848448-49
CTCGTAT96950.035.96142644-45
TCTCGTA100250.032.59813342-43
ATCTCGT109400.030.58808542-43
ATGCCGT118650.029.88482548-49
TACTCGA121550.029.67983436-37
GTATGCC122500.029.19762646-47
ACTCGAT118600.028.59579536-37
CCGTCTT126250.028.5561152-53
GCCGTCT124950.028.34001450-51
TTACTCG131200.027.17114834-35
TGCCGTC137650.026.38091950-51
CGTCTTC143750.024.8153752-53
CTCGATC135350.024.74112938-39
TCGATCT134750.023.97004138-39
GATCTCG136550.023.96713640-41
ATTACTC156500.023.53740734-35
ACATTAC163650.022.5381932-33
Produced by FastQC (version 0.11.5)