FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9580542
Sequences flagged as poor quality0
Sequence length40
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4073914.252275080052883No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT3020133.1523581859982457No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCT2686172.8037766548072125No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA2282092.382005109940544No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACAC1823651.903493560176449No Hit
GATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC1337291.3958396090743093No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCG1016401.0609003123205347No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGC942800.9840779363004725No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCC866690.904635666750378No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN744380.7769706557311684No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACG285530.29803115523109236No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAA222970.23273213561403938No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC204950.21392317887651868No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTA178870.18670133693897484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)