FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_sox2_so.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_sox2_so.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413114
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC37556790.91122547287189No Hit
CGATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAAC23140.560135943105293No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12070.29217116824895795No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACG8990.2176154765996795No Hit
CGATCGATCGACTCGGTGCCACTTTTTCAAGTTGATAACG8720.21107975038367136No Hit
CATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACG8570.20744879137477792No Hit
CGATGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACG7460.18057969470896654No Hit
CGATCATCCGACTCGGTGCCACTTTTTCAAGTTGATAACG5970.14451216855395846No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAA5790.14015501774328637No Hit
TGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC5670.1372502505361716No Hit
CGATCGATCCGACTTGGTGCCACTTTTTCAAGTTGATAAC5390.13047246038623722No Hit
CGATCAATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC4880.11812719975599956No Hit
CGATCGATCAGACTCGGTGCCACTTTTTCAAGTTGATAAC4770.11546449648281104No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAGGTTGATAAC4420.10699225879539304No Hit
CGATCGATCCGACTCGGTGCACTTTTTCAAGTTGATAACG4280.10360336372042583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)