FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_sox2_ps.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_sox2_ps.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310195
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAA28091590.56077628588469No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18210.5870500814004094No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAA10870.3504247328293493No Hit
ACGATCGATCGACTCGGTGCCACTTTTTCAAGTTGATAAC10710.3452666870839311No Hit
AGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC10030.3233449926659037No Hit
ACATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC9020.29078482889795126No Hit
ACGATGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC8750.28208062670255807No Hit
ACGATCATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC7780.2508099743709602No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC7140.23017779138928737No Hit
ACGATCGTCCGACTCGGTGCCACTTTTTCAAGTTGATAAC5470.17634068892148488No Hit
ACGTCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC5350.17247215461242124No Hit
ACGATCGATCAGACTCGGTGCCACTTTTTCAAGTTGATAA4460.14378052515353246No Hit
ACAATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAA4290.1383001015490256No Hit
ACGATCGATCCGACTTGGTGCCACTTTTTCAAGTTGATAA4100.1321749222263415No Hit
ACGATCAATCCGACTCGGTGCCACTTTTTCAAGTTGATAA3870.12476023146730283No Hit
ACGACGATCCGACTCGGTGCCACTTTTTCAAGTTGATAAC3710.11960218572188462No Hit
ACGATCGATCCGACTCGGTGCACTTTTTCAAGTTGATAAC3510.11315462854011188No Hit
ACGATCGATCCGACTCGTGCCACTTTTTCAAGTTGATAAC3190.10283853704927545No Hit
ACGATCGATCCACTCGGTGCCACTTTTTCAAGTTGATAAC3120.100581892035655No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAAGTTGGTAA3110.10025951417656634No Hit

[OK]Adapter Content

Adapter graph