FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_sox2_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_sox2_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437042
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTA40253592.10442017014383No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17550.4015632364852806No Hit
ATCCGACTCGGTGCCACTTTTTTAAGTTGATAACGGACTA14130.3233098878368669No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACTGACTA13050.29859830405315735No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACAGACTA10360.23704815555484368No Hit
ATCCGACTCGGTGCACTTTTTCAAGTTGATAACGGACTAG9380.21462468138073687No Hit
ATCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAG9110.2084467854348095No Hit
ATCAGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTA7770.17778611666613278No Hit
ATCCGACTTGGTGCCACTTTTTCAAGTTGATAACGGACTA6040.1382018204200054No Hit
ATCCGACTCGGTGCCATTTTTCAAGTTGATAACGGACTAG5570.12744770525487253No Hit
ATCCGACTCGGTGCCACTTTTCAAGTTGATAACGGACTAG5550.12699008333295198No Hit
AACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTA4820.11028688318285199No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGGGCTA4610.10548185300268624No Hit
ATCCGACTCGGTGCCACTTTTTCAAGTAGATAACGGACTA4510.1031937433930835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)