FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_old_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_old_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6074729
Sequences flagged as poor quality0
Sequence length40
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCC564835192.9811189931271No Hit
ACGATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCG105260.17327521935546425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102010.16792518645687735No Hit
ACGATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCC79880.1314955778274224No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGATCC71250.11728918277671317No Hit
ACGATCGATCCGACTCGATGCCACTTTTTCAACTAGTTCC63130.10392233135008985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)