FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_old_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_old_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6753411
Sequences flagged as poor quality0
Sequence length40
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCG628354793.04256767432044No Hit
CGATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGC104430.15463296991697972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94070.13929257378234494No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCC81230.12027995926799065No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGATCCG80970.11989496863140715No Hit
CGATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCG72760.10773814891467438No Hit
CGATCGATCCGACTCGATGCCACTTTTTCAACTAGTTCCG68110.10085273945270028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)