FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_old_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_old_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934039
Sequences flagged as poor quality0
Sequence length40
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT558195794.06673936588554No Hit
TCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTA93340.15729589913379402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76140.12831058238747672No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTA72480.12214277661471386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)