FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_ASD_CRISPRi.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_ASD_CRISPRi.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516137
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACG47048391.15467404971935No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34820.6746270854443684No Hit
GATGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGG15160.2937204656903109No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGG14830.2873268143923028No Hit
GATCGATCGACTCGGTGCCACTTTTTCAAGTTGATAACGG14740.2855830913110279No Hit
GATCATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGG11750.227652735610894No Hit
GTCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGG8660.16778490982045463No Hit
GATCGATCCGACTCGGTGCCACTTTTTTAAGTTGATAACG8470.16410371664887424No Hit
GATCGATCCGACTTGGTGCCACTTTTTCAAGTTGATAACG7640.14802271489933874No Hit
GATCGTCCGACTCGGTGCCACTTTTTCAAGTTGATAACGG6780.13136047212271162No Hit
GATCGATCCGACTCGGTGCACTTTTTCAAGTTGATAACGG6400.12399808577955077No Hit
GACGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACGG6240.12089813363506201No Hit
GATCGATCAGACTCGGTGCCACTTTTTCAAGTTGATAACG6190.11992939858990928No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAAGTTGATAACA5610.10869207206613749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)