FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11229646
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGG373950.33300248289215884No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA306660.2730807364720134No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT289410.25771961110795477No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAA269280.23979384568311415No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT265800.23669490560966924No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATAC256480.22839544541297208No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACT232290.20685424990244572No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAA224450.19987272973698372No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATT224020.1994898147279086No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG222410.1980561096939298No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTG220540.19639087465446373No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG207720.18497466438389956No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTT196760.17521478415259037No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA192040.17101162405297549No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA191230.17029031903588054No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGG190520.16965806402089612No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGT188940.16825107398755046No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAA187730.16717356896201357No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC187250.16674612895188326No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA185640.16531242391790443No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAG179800.16011190379465212No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGG179380.15973789378578807No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCAC177840.1583665237532866No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACAT175020.15585531369377092No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT174700.15557035368701738No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT174210.155134008676676No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTT173230.15426131865599327No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACA170470.15180353859774387No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTT165220.1471284134869434No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTC164640.1466119234747026No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGT164300.14630915346752696No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGA162680.14486654343333707No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCA157970.14067228833393322No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAA152410.13572110821659025No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAA152170.1355073882115251No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTC152070.13541833820941462No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCT148760.13247078313955757No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA148270.1320344381292162No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT145170.12927388806379114No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAA144640.12880192305260557No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC144360.12855258304669623No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGG143500.12778675302854606No Hit
GGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGG142350.1267626780042755No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGA142150.12658457800005452No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGG141700.12618385299055732No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGA140890.12546254797346237No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCA140210.12485700795911109No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG139870.12455423795193545No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTA139330.12407336794053882No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACAT138600.12342330292513229No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGG138190.1230581979164793No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTAT137870.12277323790972573No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG137650.12257732790508268No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTC137600.12253280290402742No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGG136100.12119705287237015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135290.12047574785527522No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGA134950.12017297784809958No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACC134870.12010173784641118No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCT134580.11984349284029079No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT134210.11951400783248199No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAAC131530.11712746777592098No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAG131460.11706513277444364No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACA129890.11566704774130902No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC129390.11522179773075661No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCA128310.11426005770796338No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAA128150.1141175777045866No Hit
AGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTA128130.1140997677041645No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAAC127710.11372575769530045No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTC127520.11355656269129054No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACA127410.11345860768896901No Hit
GGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTAT126360.11252358266680891No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAG126130.11231876766195481No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAA125510.1117666576488698No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTC125180.11147279264190517No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGC124910.11123235763620687No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA124880.11120564263557373No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACT124790.11112549763367428No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGA123440.10992332260518274No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGAC121110.10784845755600844No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTAC120860.10762583255073223No Hit
TCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTT120550.10734977754418971No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGAC119250.10619212751675342No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATG118920.10589826250978882No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTT115610.10295070743993177No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTC115440.10279932243634393No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTC115320.10269246243381136No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCT114860.10228283242410313No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGA113960.10148138240510876No Hit
GATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGA113770.10131218740109885No Hit
GTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGC113670.10122313739898835No Hit
CTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAGA113380.10096489239286796No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTT112720.10037716237893875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)