FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_A5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_A5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11821021
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT287480.24319388316795987No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT257840.21811990690144278No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAA244130.20652192395225422No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA230680.19514388816329825No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT224400.18983131829306452No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATAC207250.17532326522387534No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAA203770.17237935707922353No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAA197520.1670921657274782No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG196910.16657613585154785No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAG194390.16444434029852412No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGG182990.15480050327294062No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGG182860.15469052969282435No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTC177630.15026620796968385No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTT175140.1481597909351485No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA173720.14695854106003195No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTT168740.14274570699096126No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA167860.14200127044863553No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA167540.1417305662514262No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGT164210.13891355069921626No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGT161020.13621496823328544No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG148970.12602126330712043No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGA148300.12545447639421334No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC146500.12393176528491068No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAA146100.12359338503839896No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGAC144150.12194378133665441No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAA144020.12183380775653813No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC141940.12007423047467729No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT140870.11916906331525846No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCT140050.11847538380990949No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGA139650.11813700356339776No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGG137780.11655507591095558No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGG136490.11546379961595533No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTG136000.11504928381397851No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG135200.1143725233209551No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTC131110.11091258530037296No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGA128440.10865389715490736No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTC125820.1064375065402557No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATT123320.10432262999955756No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA123150.10417881839479011No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT121940.10315521814909219No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACAT121510.10279145938409211No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAA119260.10088807049746379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)