FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6742708
Sequences flagged as poor quality0
Sequence length40
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT224180.3324776929388014No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT211380.31349422220271145No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA210810.31264886452149493No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAA208730.3095640505268803No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATAC177450.2631731939155604No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGG171200.2539039210952039No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAA159770.23695227496133603No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAG158280.234742480320963No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT156250.23173182050891125No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA156060.2314500346151724No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAA155150.23010042849252854No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG151810.22514692909733006No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGG144760.21469118935596795No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGT143160.2123182555139567No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTT139900.20748340281085878No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTT139470.20684567684081828No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA136600.20258922676171057No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA136590.202574395925198No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTC132630.19670138466622014No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATT127070.18845543956523106No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGT125960.18680921671233575No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG122990.1824044582681024No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG120770.17911201256231177No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTG118540.17580473602000857No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAA118160.1752411642325309No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC116990.17350595636056018No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC115780.17171142514253918No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACAT113610.16849313361931142No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA113340.16809270103347201No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT111740.16571976719146075No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT111690.1656456130088979No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGAC110880.1644443152513797No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGA110430.16377692760831405No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGA109760.16278326156197184No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAA107700.15972810924038236No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACT106640.1581560405700499No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGG106000.15720686703324538No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTC105040.15578310672803866No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGA104980.1556941217089632No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAA103440.1534101728860274No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTC103030.15280210858901203No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCA101470.15048849809305104No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCT99640.14777445501125067No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACA99430.1474630074444867No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGG97460.14454133265151034No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG96260.1427616322700019No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACA95460.14157516534899628No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCA94270.13981029580400042No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTA94100.1395581715832867No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTT92780.13760050116362743No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTC92180.13671065097287322No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA91960.13638437256959665No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTG91770.13610258667585784No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCA91580.135820800782119No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGA91050.13503476644695275No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAG90920.13484196557228936No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGA90190.13375931450687173No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAAC89940.13338854359405747No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAG88890.13183130576023758No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGG88570.13135671899183532No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCT88180.1307783163678451No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTAT85590.12693712971108936No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGG84490.12530573769470663No Hit
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGT84260.12496462845491751No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAA84180.12484598176281696No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGG83300.12354086814971078No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTG83060.12318492807340908No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCT83020.12312560472735881No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAA82920.12297729636223309No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGA82780.12276966465105711No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATC82720.12268067963198169No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGC82180.12187981446030288No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCA82010.1216276902395892No Hit
AGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTA81530.12091581008698582No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACC81100.1202780841169453No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTT80580.11950688061829165No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCAC80340.11915094054198996No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAG79030.11720810095884324No Hit
GGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGG78720.11674834502695355No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTG78560.11651105164275244No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTT78250.11605129571086277No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACA77680.11520593802964625No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAG77580.11505762966452056No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGG77530.1149834754819577No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCAC77530.1149834754819577No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGAC77440.11484999795334455No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCAT77380.11476101293426913No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAA77030.11424193365632918No Hit
TCTATTGATGAGGGTCTTACTCTTTTAGTATAAATAGTAC76910.11406396361817835No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACT76900.11404913278166577No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTC76040.1127736808415847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75900.11256604913040874No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTC75610.1121359548715442No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATC75410.11183933814129278No Hit
AGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGG75350.11175035312221736No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACAT75170.11148339806499111No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAAC74930.11112745798868941No Hit
AGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCC74890.11106813464263914No Hit
ACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATAAC74650.11071219456633744No Hit
GCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTG74430.11038591616306088No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAA74280.11016345361537234No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATG73150.1084875690894519No Hit
ACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAAC73000.10826510654176333No Hit
GTAAGGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAA71820.10651506783328005No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT71820.10651506783328005No Hit
CCTCAACTGCCTGCTATGATGGATAAGATTGAGAGAGTGA71730.1063815903046669No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCC71390.10587734186323951No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTA70110.10397899478963052No Hit
CGCGGGCGGGGGATATAGGGTCGAAGCCGCACTCGTAAGG69910.1036823780593791No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTAC69700.10337093049261513No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT69650.10329677631005227No Hit
GTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGC68040.10090901163152848No Hit
GTCGTAAGCCTCTGTTGTCAGATTCACAATCTGATGTTTT67830.10059756406476449No Hit
GCTTACGACCCCTTATTTACCGAGAAAGCTCACAAGAACT67640.10031577817102563No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC67460.10004882311379938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)