FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15926490
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT514610.3231157649927887No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT496110.3114998973408454No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA436210.27388960153806646No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAA426250.2676358695481553No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATAC402500.25272360702201174No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG386180.24247652809878387No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGG382200.23997754684177117No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAA377730.23717090206316643No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGG340590.21385126289596765No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA337180.2117101759395824No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA336980.21158459899199383No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAA329750.20704499233666676No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT324220.2035727897358426No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAG318750.20013826021929504No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTT318670.2000880294402596No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTC292680.18376930510112396No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG285320.17914807342986433No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAA272470.17107975454729824No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTT271810.17066535062025592No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA270250.16968585042906503No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGT264920.16633922477582944No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGT259970.16323119532301217No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACAT245070.15387571272766315No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACT243210.1527078471150894No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGAC242620.1523373951197031No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT241050.15135161608113276No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA239180.15017747162117956No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGG236040.1482059135440389No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATT233090.1463536535671074No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC233050.14632853817758967No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG231800.14554368225516107No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC229770.144269076237137No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT229030.14380444153105926No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTG225620.14166335457467402No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGA224980.14126150834239057No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGA211730.13294203556464732No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAA211510.1328039009222999No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTC205280.1288921790049157No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTG204820.12860335202546197No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAA204130.12817011155628139No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGA204000.12808848654034882No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTT202780.1273224671600585No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTC200420.12584065917851328No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACA200100.12563973606237155No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAG199860.1254890437252653No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCA196280.12324121636342973No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGG193990.12180336031354053No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTC192620.12094315822255876No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGA192330.12076107164855535No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTG192200.12067944663262276No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGC192090.12061037931144904No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG189150.11876439818189696No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACA188630.11843789811816666No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGG188260.11820558076512779No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTA187500.1177283883642912No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGA186510.11710678247372774No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAA185870.11670493624144429No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCT184650.11593891686115397No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAAC182170.1143817627110556No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA182100.1143378107793996No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTT181750.11411805112111958No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCAC178790.11225951229680865No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAA177660.1115500025429332No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCA176620.1108970024154726No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGG176100.11057050235174229No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTC175840.11040725231987714No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCT174320.10945286751820393No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT173240.10877475200122563No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCA172910.10856755003770449No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTAT172640.10839802115845991No Hit
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGT172000.10799617492617646No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGA168660.10589903990144722No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCAC168470.10577974180123806No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGG167660.10527115516350433No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAG167560.10520836668971004No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAG167240.10500744357356832No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATC166390.10447374154631686No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAG165700.10404050107713626No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAA162840.10224475072661962No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTG162740.10218196225282533No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC159640.10023551956520238No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATG159540.10017273109140809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)