FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268943
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT80730.3558044428617202No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT73520.32402753176258725No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAA70110.3089985072344259No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA70010.30855777337729506No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG58460.25765301287868403No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAG55140.24302064882194044No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT54850.241742520636261No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAA53870.2374233288363789No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATAC53820.23720296190781345No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA51600.227418670279509No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAA50860.22415723973674084No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGG49420.2178106721940569No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGG49170.2167088375512298No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTC47260.20829082088003092No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTT46850.20648381206579453No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA45780.20176795979449463No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTT45390.20004909775168436No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGT44680.19691988736605545No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGT44570.19643508012321154No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA43410.1913225673804939No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGAC42790.18859001746628276No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG40630.1790701661522568No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC40100.1767342767094634No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGA39700.17497134128094008No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG39110.17237101152386816No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC38350.16902143420967386No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA38300.1688010672811084No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT37880.16694998508115894No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAA37420.16492260933835712No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAA35910.15826752809568156No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT34900.15381611613866017No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAA34570.15236169441012842No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACAT34400.151612446853006No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTG34280.151083566224449No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCT33950.14962914449591727No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGA33940.1495850711102042No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACT33780.14887989693879486No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATT33690.1484832364673771No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTG32750.14434033821034728No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCA32550.14345887049608563No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAG32540.14341479711037253No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTC32020.1411229810532922No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTG31810.14019743995331746No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCA31710.13975670609618662No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG31640.13944819239619505No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGG31610.1393159722390558No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGG31080.1369800827962624No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAA30800.1357460279962961No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACA30760.13556973445344372No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGA30160.13292533131065876No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTC30160.13292533131065876No Hit
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGT29970.1320879369821102No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAG29680.13080980879643075No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTG29370.1294435338393252No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATC29190.12865021289648967No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGG29130.12838577258221118No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCAC29050.12803318549650652No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGA28990.12776874518222803No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTC28980.12772467179651495No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTA28860.12719579116795796No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTT28820.1270194976251056No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAAC28600.12604988313941778No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCA28370.12503619526801688No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACA28280.12463953479659913No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTC28010.12344955338234587No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGC27990.12336140661091971No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCAT27840.12270030582522345No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTT27750.12230364535380572No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGG27750.12230364535380572No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATA27660.12190698488238796No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCC27130.11957109543959456No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTAT26970.11886592126818522No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAA26750.1178963067824974No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCT26380.11626559151111332No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGA25870.11401784883974608No Hit
AGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGG25820.11379748191118068No Hit
TCTATTGATGAGGGTCTTACTCTTTTAGTATAAATAGTAC25710.11331267466833676No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGA25660.11309230773977133No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATC25610.11287194081120591No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCA25480.11229898679693585No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAG25320.1115938126255265No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATG25170.11093271183983026No Hit
AGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCC25050.11040383121127327No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACT24980.11009531751128168No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGG24890.10969865703986395No Hit
ATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAG24700.10886126271131535No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTT24450.10775942806848827No Hit
ACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAAC23810.10493873138285095No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACC23770.10476243783999863No Hit
AGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTA23740.10463021768285936No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAA23560.10383689674002387No Hit
GCTTACGACCCCTTATTTACCGAGAAAGCTCACAAGAACT23560.10383689674002387No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCT23560.10383689674002387No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTA23490.10352838304003231No Hit
GTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGC23450.10335208949717996No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACAT23440.10330801611146687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23420.10321986934004072No Hit
GGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAA23370.1029995024114753No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC23250.10247062178291831No Hit
GCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTG23110.10185359438293513No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT23060.10163322745436973No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACA23040.10154508068294354No Hit
CCTCGGAGCTGGTAAAAAGAGGCCTAACCCCTGTCTTTAG23020.1014569339115174No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAG22930.10106027344009964No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGG22910.10097212666867346No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTC22880.10083990651153422No Hit
GGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGG22860.10075175974010805No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGT22790.1004432460401165No Hit
CGCGGGCGGGGGATATAGGGTCGAAGCCGCACTCGTAAGG22740.10022287911155105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)