FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8529156
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAAT240480.28195052359225226No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGA205570.24102033073377951No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT202310.23719814715547471No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAA200520.23509946353425826No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGG163950.1922230054181211No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAA161540.1893974034476565No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT159310.18678284228826392No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAA146250.17147065899603664No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAG145040.17005199576605234No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATAC133750.15681504711603353No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGG128630.15081210848998425No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGG127880.14993277177718403No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA127380.14934654730198393No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTC127000.14890101670083183No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGT126620.14845548609967973No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTT124890.1464271494154873No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA120920.14177252708239832No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATAC120380.14113940464918218No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCT119360.13994350671977393No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTT119150.13969729244018986No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAG116670.13678961904319725No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGAC115880.13586338437238105No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATA113340.1328853640383644No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAG110500.1295556090192277No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCATCTC108090.1267300070487631No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGA105750.1239864765048265No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGT105530.12372853773573846No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAA105500.12369336426722644No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC103700.121582956156506No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTG100700.11806560930530524No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGA99130.11622486445317685No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT97150.11390341553138435No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCA92680.10866256872309522No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTG92640.10861567076507922No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCAT92120.10800599731087107No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAA91650.10745494630418297No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAA90550.10616525245874268No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACA90270.10583696675263063No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTG90190.10574317083659861No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCAC89720.10519211982991049No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCA89150.10452382392818235No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACT88920.10425416066959028No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTC87770.10290584437663No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTT86450.10135821176210168No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCA86300.10118234441954163No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGG85870.10067819137086953No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAA85630.10039680362277346No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGG85340.10005679342715738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)