FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_145_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_145_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299227
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACAC301816691.48100448983959No Hit
TCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACA108020.3274100266516975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107780.32668258352638363No Hit
TCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACACA67710.20522989172918385No Hit
CCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACA57880.17543503372153538No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACAA54740.16591765283201187No Hit
TCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTTACACA50590.1533389487901257No Hit
TCCGACTCGTGCCACTTTTTCAACTAGTTCCGCTTACACA49150.1489742900382423No Hit
TCCACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACA41370.12539300872598338No Hit
TCCGACTGGTGCCACTTTTTCAACTAGTTCCGCTTACACA40300.12214982479229225No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCAGCTTACAC35050.1062370064260507No Hit
TCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACAC33600.1018420375439459No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTACGCTTACAC33460.10141769572084612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)