FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_145_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_145_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4519077
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCT406663189.98808827554831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310100.6862020717947492No Hit
ATCGATCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT157610.34876590949877595No Hit
ATGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT130690.2891962230340399No Hit
ATCATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT102150.22604173374341707No Hit
ATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTT89140.19725266907379538No Hit
ATCGATCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTT86900.1922959046725692No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT74650.16518859935336352No Hit
ATCGATCCGACTCGTGCCACTTTTTCAACTAGTTCCGCTT71780.15883774496429248No Hit
ATCGTCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT66430.1469990442738639No Hit
ATCGATCCACTCGGTGCCACTTTTTCAACTAGTTCCGCTT61010.13500544469589698No Hit
ATCGATCCGACTGGTGCCACTTTTTCAACTAGTTCCGCTT59150.1308895599698788No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGTT49840.1102880079272825No Hit
ACGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT49760.1101109806272387No Hit
ATCGATCCGACTCGGTGCCATTTTTCAACTAGTTCCGCTT47840.1058623254261877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)