FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_145_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_145_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469332
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTT405793090.7950002371719No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190050.42523133210958597No Hit
TCGATCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA147890.3308995617242129No Hit
TGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA117700.2633503172286149No Hit
TCATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA92530.2070331763225466No Hit
TCGATCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTTA91230.20412446423760866No Hit
TCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTA84550.1891781590626966No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA83070.18586670222753646No Hit
TCGTCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA69060.15451973583524337No Hit
TCGATCCGACTCGTGCCACTTTTTCAACTAGTTCCGCTTA67640.1513425272501573No Hit
TCGATCCACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA58090.12997468078003602No Hit
TCGATCCGACTGGTGCCACTTTTTCAACTAGTTCCGCTTA57650.12899019361282626No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTA53350.11936906902418527No Hit
TCGATCCGACTCGGTGCCATTTTTCAACTAGTTCCGCTTA51220.11460325614655613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)