FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_145_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_145_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4162161
Sequences flagged as poor quality0
Sequence length40
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTA381267491.60323207103234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104730.25162409623270215No Hit
CGATCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC94590.22726175176789173No Hit
GATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC77090.18521628548246932No Hit
CGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTAC73850.17743186772448255No Hit
CGATCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTTAC66300.15929225226991459No Hit
CATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC61540.1478558854402797No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTACGCTTA53950.12962016606277363No Hit
CGATCCGACTCGTGCCACTTTTTCAACTAGTTCCGCTTAC44890.10785262751729209No Hit
CGTCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC42180.10134158673823525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)