FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n02_145_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n02_145_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3814467
Sequences flagged as poor quality0
Sequence length40
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC349775191.69697889639627No Hit
GATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAA119730.3138839580995196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114100.299124359969558No Hit
GATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACA70600.18508483623006833No Hit
GATCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACA60350.1582134541994989No Hit
GATCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTTACA50280.13181395985336877No Hit
GATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTAC49950.13094883243189676No Hit
ATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACA47620.12484050851665515No Hit
GATCCGACTCGGTGCCACTTTTTCAACTAGTTACGCTTAC43600.11430168356417816No Hit
GATCCGACTCGGTGCCACTTTTTCAACTAGTTCAGCTTAC43170.11317439631801768No Hit
GATCCGACTCGGTGCCATTTTTCAACTAGTTCCGCTTACA41360.10842930349115618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)