FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9580542
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA7639367.973828620551949No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC6355726.63398793095422No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC5712285.962376658857087No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC5410205.64707090684431No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC98070.1023637284821673No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)