FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_sox2_so.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_sox2_so.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413114
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT13870.33574267635567906No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGGGGTCGAGCGTTGCG8520.20623847170514678No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGCACTCACACACATCG8400.20333370449803204No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTTTGTTTCCGCGGCTC8390.20309164056410578No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTTGCTCGCGAGGAGTG8260.1999448094230648No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCCTCAAGACTCCACGC7770.18808367666067963No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGCAGGCTAGATGACAC7680.18590510125534354No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCAATCCAGCAGTTGC7560.18300033404822882No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAAGGGCGTGCCCTGCGT7300.17670667176614688No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGTGCCCGCTGCGCTGC7030.1701709455501387No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACCCGCGACCCGGAGCA6850.16581379473946659No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTCCTCCTCAGCAGCAC6620.16024632425916333No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGGCGGGATCAGCACCG6400.15492091771278632No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCCCCGGGCCTGGCTCC6050.14644868002536832No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTCGCATTTCCCGCAGC6040.1462066160914421No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGGTGGCGGGATCAGCA5970.14451216855395846No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGGCTCTGGTAGGAAGC5960.14427010462003226No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGCTCGGTACCACCCGG5900.14281772101647489No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGATGCCCCTCTCGAGA5890.14257565708254866No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCACTGCCCGACTCCGC5870.14209152921469617No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGGAAAGAGCTAGATGG5820.14088120954506503No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATAAGCTACTCTGAGTT5710.13821850627187654No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCTCGTAGTACTTCACG5530.13386135546120442No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGGACGGGAGGCGAGCA5170.1251470538398602No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGCCCTCGTCGCAACC5090.12321054236845036No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGAACATGGCGCACAGC5090.12321054236845036No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTTCTCTCTCTCTCCAA5090.12321054236845036No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGAACGCGGGCCGAGCT5010.12127403089704052No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCCTCCTCCGCTCGGCG4980.12054783909526184No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG4920.11909545549170447No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCAGTGGAGGCAGAGG4870.11788513582207333No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCGGCAGCGGCGGTTC4790.1159486243506635No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGGCGATGCTTCAGGTG4780.11570656041673727No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACCGGGAAGCTGGAGGG4610.1115914735399914No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGCCGCTCCCCTCAGCC4600.11134940960606514No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGCGACCCGGAGCAGCT4580.1108652817382127No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACTATCATGGCACCCAA4580.1108652817382127No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTCCATGTGTGCGGCC4520.10941289813465532No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGCTGGTGTAGCTTGGA4510.10917083420072911No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCGGGCCGTGGGGAGCC4490.10868670633287664No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT4410.10675019486146682No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCATGGCCCAGTCCTCC4400.10650813092754059No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCGCGGTGCTGAAGCCC4330.10481368339005698No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGAGCCCGGAGCCCATT4320.10457161945613075No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGCGAGAGGTGGATCG4270.1033612997864996No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAAGAAGCGCAGCGCCGG4260.10311923585257339No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGACGGAAAAGAGCGAAAG4250.10287717191864716No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCACGCGGGAAAAGCTT4220.10215098011686846No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGTGTGTGTGCGTGCGA4160.1006985965133111No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGGAGGCCGCGGGCCGG4150.10045653257938487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)