FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_sox2_ps.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_sox2_ps.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310195
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTGATCCGTAGTTACA5290.1705378874578894No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGACAGTCACAGCCATC4870.1569980173761666No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGACAGTCACAGCCATCT4670.15055046019439383No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCAATCCAGCAGTTGC4360.14055674656264608No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTTTGTTGCCTGAGGT3990.12862876577636648No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGTGTTTGTGTGCTAGGC3770.12153645287641644No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCGGCAGCGGAGGCAG3690.11895743000370734No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTATCCACCTCGATGAC3590.11573365141282098No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAGAGCTAGATGGAGCA3580.11541127355373232No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCTCCTCCTCCTCCTCC3570.11508889569464369No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAGAGCTAGATGGAGCAG3560.11476651783555505No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGAGGCGCCAGTACCCG3510.11315462854011188No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTGTTTGTGTGCTAGGCT3500.11283225068102322No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAGCCGGCTTGTGACAGT3380.10896371637195956No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGTCTGATCCGTAGTTAC3280.1057399377810732No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGAGCGCCCGCCTGTTC3250.10477280420380729No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGACAGTCAC3190.10283853704927545No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGGGAAGATGGCGGCTG3160.10187140347200956No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTGAGTGTTTCCAAGT3130.10090426989474363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)