FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_sox2_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_sox2_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437042
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGCCCCGGGCCTGGCTCC7230.165430324774278No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGCCCCGGGCCTGGCTC6600.15101523423378072No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCCCCGGGC5740.13133749159119718No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGGGCTCTGGTAGGAAGC4550.10410898723692459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)