FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_old_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_old_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5014134
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA125259624.98130285309487No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC116074923.1495408778465No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC108422321.62333515618051No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC108173221.573655590377122No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT123470.24624391769346413No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT93030.18553552816897195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)