FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_old_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_old_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934039
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA151986725.612689771671537No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC133952322.573545606963485No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC128359721.63108466257131No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC126977921.398224716757003No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT150760.2540596716671394No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT111500.18789900100083604No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCAC61890.10429658450171965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)