FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_ASD_CRISPRi.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_ASD_CRISPRi.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516137
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAGCGGCAGCGGCGGTTC6610.12806677296919228No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCCGCTGCCGCTC5940.11508572336414558No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCCTCCTCCTCCTCCTCC5510.10675460197583199No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGAGGCCTCGGGAGCGG5480.10617336094874034No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTGCTGGGGCCGCGGCGG5270.10210467375909883No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCACTCAGCCCGCTGCCC5260.10191092675006828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)